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Olympus Motorized Multicolor TIRF for Simultaneous Acquisition

Olympus Motorized Multicolor TIRF for Simultaneous Acquisition

Description


Olympus, the world leader in objective-based TIRF microscopy, announces another significant leap forward in multicolor TIRF. The Olympus cell^TIRF illuminator offers four motorized channels for simultaneous image capture. Intuitive software control of TIRF parameters allows instant setting and confirmation of TIRF angle and seamless transition back and forth to widefield fluorescence. With the cell^TIRF illuminator, each laser wavelength is optimally focused and each angle is individually set, allowing different wavelengths to have the same penetration depth. Users can preset calculated penetration depths for all lasers with a single mouse click; the system will individually adjust each laser’s angle to simultaneously capture TIRF information from all channels.

Features and Benefits

Feature Image
  • The only 4 line system with true simultaneous acquisition
  • Motorized control of TIRF angles
  • Precise adjustment of incident angle
  • Software calculation of penetration depth
  • Software based laser attenuation
  • Integrated Point FRAP optics for 1st laser line
  • Seamless widefield/TIRF switching
  • 6 dedicated TIRF lenses, world’s highest NA of 1.65
  • 405 – 640nm laser systems
    • Flexibility to select your optimal wavelength
  • TIRF and widefield or 100% widefield with 340nm Transmission
  • 25mm filter access for each laser line
  • Adjustable field stop for each laser line
  • Rigid single billet Aluminum frame
  • Single molecule and membrane research
  • Feature Image

    Specifications

    TIRF illuminator

    Available laser Systems

    405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm

    Laser coupling

    FC or FC8 connector

    Field Number

    FN11.8

    Illuminator Body

    Single Billet Aluminum

    Safety

    Integrated Interlock

    Laser systems

    Wavelengths

    405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm

    Power

    Laser system available up to 100mW

    Attenuation

    Software control of laser intensity

    Imaging Shutter

    High speed 1msec TTL shutters

    Safety

    Integrated interlock

    Epi-Fluorescence

    Light source

    100W Mercury, 75W Xenon, Metal halide.

    Selection

    TIRF/Brightfield or Brightfield only with 340nm transmission

    Field number

    FN22

    TIRF Objectives

    60X Objectives

    PLANAPON60XOTIRFM

    PLANAPO 60X, NA 1.45, WD 0.1mm, Correction collar

    APON60XOTIRFM

    APO 60X, NA 1.49, WD 0.1mm, Correction collar

    100X Objective

    PLAPO100XOTIRFM

    PLANAPO 100X, NA 1.45 WD 0.10mm

    UAPON100XTIRF

    UAPON 100X, NA 1.49 WD 0.09mm, Correction collar

    AP0100XO-HR-SP

    APO 100X, NA 1.65, WD 0.10mm

    150X Objectives

    UAPON150XOTIRF

    UAPO 150X, NA 1.45,WD 0.07mm,Correction collar

    Immersion Oil

    Type-F

    Low Auto Fluorescence Immersion oil

    High Refractive Index

    RI 1.78 Immersion oil for use with AP0100XO-HR-SP 1.65NA 100X objective

    Coverslips

    High Refractive Index

    RI 1.78 glass coverslips for use with AP0100XO-HR-SP 1.65NA 100X objective

    Compatible microscopes

    IX71, IX81 and IX81-ZeroDrift

    Compatible microscopes IX71, IX81 and IX81-ZeroDrift

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